FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000108482
Interaction Network
Interactions Browser links
ttk network
MEP-1 network
Reported Interactions
FBrf0212010-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ttk 

ttk

bait

fused to LexA DNA-binding domain

ttk 

MEP-1

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ttk 
interaction domain
sufficient binding region
aa 392-500

coordinates relative to ttk-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast LexA-BD/GAL4-AD

FBrf0212010-11.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

MEP-1

bait
ttk 

ttk

prey
ttk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0212010-2.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

MEP-1

bait

GST tag

ttk 

ttk

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0212010-38.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

MEP-1

bait
ttk 

ttk

prey
ttk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0249384-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ttk 

TTK

bait

fused to GAL4 DNA-binding domain

ttk 
ttk 

Mep-1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
interaction domain residues 250-349
sufficient binding region
aa 250-349

coordinates relative to MEP-1-PA

interaction domain residues 942-1152
sufficient binding region
aa 250-349

coordinates relative to MEP-1-PA

interaction domain residues 1083-1152
mutation disrupting interaction
aa 1083-1152

deletion within the context of aa 942-1152.

ttk 
interaction domain residues 500-642
sufficient binding region
aa 500-642

coordinates correspond to the ttk88 isoform.

ttk 
interaction domain residues 392-500
sufficient binding region
aa 392-500

coordinates correspond to the ttk69 isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The same region in the TTK isoforms ensures the interaction with both regions of MEP-1. The ttk69 interaction domain was confirmed from previous publication.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)