FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000108687
Interaction Network
Interactions Browser links
ft network
ND-24 network
Reported Interactions
FBrf0226170-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ft 

ft

bait

fused to GAL4 DNA-binding domain

Ndufv2

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Screen originally performed in Fanto et al., 2003 (FBrf0158896) but reported here. Positives subjected to tests for genetic interaction with ft.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0226170-4.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ft 

Ft

bait

GST tag

ft 
ft 

Ndufv2

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ft 
highly conserved regions 1 and 2
sufficient binding region

"1-2" construct, coordinates not reported

ft 
highly conserved region 2
necessary binding region
SDIDIVYHYKGYREA

"N-2Δ15" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

A soluble 68 kDa fragment of ft is cleaved and transported to mitochondria where it binds to ND-24.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)