Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

300 Cam clones recovered in this igl two hybrid screen.

Two-hybrid system: yeast LexA-BD/B42-AD

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Phosphomimetic mutation of both igl PKC phosphosites (T177 and S227) disrupts binding of igl to Cam, suggesting that Cam does not bind phosphorylated igl.