FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Kap3, Klp64D
FlyBase ID
FBig0000108801
Interaction Network
Interactions Browser links
Kap3 network
Klp64D network
Reported Interactions
FBrf0209149-10.PD.WB
Reference
Doodhi et al., 2009
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Kap3 

DmKAP

bait

His or GST tag

Kap3 
Klp64D 

KLP64D

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Kap3 
N-terminal domain
sufficient binding region
aa 94-494
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Doodhi et al., 2009)
FBrf0209149-13.CH.WB
Reference
Doodhi et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

KLP64D

bait

His tag

Klp64D 
Kap3 

DmKAP

prey

GST or no tag

Kap3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Kap3 
N-terminal domain
sufficient binding region
aa 1-631aa 94-828
Klp64D 
stalk domain
sufficient binding region
aa 425-589
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Doodhi et al., 2009)
FBrf0209149-17.CH.MW
Reference
Doodhi et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Kap3 

DmKAP

bait

GST tag

Kap3 
Klp64D 

KLP64D

prey

His tag

Klp64D 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Kap3 
N-terminal domain
sufficient binding region
aa 94-828
Klp64D 
stalk domain
sufficient binding region
aa 425-589
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Doodhi et al., 2009)
FBrf0209149-5.CS.WB
Reference
Doodhi et al., 2009
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Kap3 

DmKAP

neutral component
Kap3 

DmKAP

neutral component
Kap3 

DmKAP

neutral component
Klp64D 

KLP64D

neutral component
Klp64D 

KLP64D

neutral component
Klp64D 

KLP64D

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Doodhi et al., 2009)
FBrf0209149-8.CH.WB
Reference
Doodhi et al., 2009
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

KLP64D

bait
Kap3 

DmKAP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Doodhi et al., 2009)
FBrf0218568-5.PD.WB
Reference
Sadananda et al., 2012
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

KLP64D

bait

His tag

Klp64D 
Kap3 

DmKAP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Klp64D 
tail fragment
sufficient binding region
aa 590-677
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from adult heads of transgenic fly line.

(Sadananda et al., 2012)
FBrf0234587-2.coIP.WB
Reference
Girotra et al., 2017
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

KLP64D

bait

His tag

Kap3 

DmKAP

prey

RFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Girotra et al., 2017)
FBrf0240954-6.PD.WB
Reference
Vuong et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Kap3 

Kap3

bait

GST tag

Klp64D 

Klp64D

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Vuong et al., 2018)
FBrf0240954-7.PD.WB
Reference
Vuong et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

Klp64D

bait

GST tag

Kap3 

Kap3

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Vuong et al., 2018)
FBrf0244469-2.PD.WB
Reference
Ahmed et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

KLP64D

bait

His tag

Klp64D 
Kap3 

DmKAP

prey

GST tag

Kap3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Klp64D 
stalk domain
sufficient binding region
aa 425-589
Kap3 
interaction domain
sufficient binding region
aa 1-399

coordinates relative to Kap3-PH

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

This interaction involved a Klp64D-Klp68D heterodimer as bait and was captured as two binary interactions, Klp64D-Kap3 and Klp68D-Kap3.

(Ahmed et al., 2020)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Ahmed et al., 2020)
FBrf0244469-4.PD.WB
Reference
Ahmed et al., 2020
Description
physical association
Assay
Collection
Source/Stage
organism / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

KLP64D

bait

His tag

Klp64D 
Kap3 

DmKAP

prey

RFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Klp64D 
stalk domain
sufficient binding region
aa 425-589
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

This interaction involved a Klp64D-Klp68D heterodimer as bait and was captured as two binary interactions, Klp64D-Kap3 and Klp68D-Kap3.

(Ahmed et al., 2020)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic larval extract.

(Ahmed et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)