Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/B42-AD

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

Ubx and DIP1 form heterodimers that bind DNA; DIP1 alone does not bind the DNA probe tested; Ubx alone binds DNA more weakly than the Ubx-DIP1 heterodimer.

Various combinations of Ubx and DIP1 were mixed with [32]P labeled DNA. DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions.