FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Dap160, nwk
FlyBase ID
FBig0000108909
Interaction Network
Interactions Browser links
Dap160 network
nwk network
Reported Interactions
FBrf0204925-2.PD.WB
Reference
O'Connor-Giles et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dap160 

Dap160

bait

GST tag

Dap160 
nwk 

Nwk

prey

RFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dap160 
SH3c-d domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic adult extract.

(O'Connor-Giles et al., 2008)
FBrf0204925-4.Y2H
Reference
O'Connor-Giles et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nwk 

Nwk

bait

fused to GAL4 DNA-binding domain

Dap160 

Dap160

prey

fused to GAL4 activation domain

Dap160 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dap160 
SH3 domains
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(O'Connor-Giles et al., 2008)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(O'Connor-Giles et al., 2008)
FBrf0205746-1.PD.WB
Reference
Rodal et al., 2008
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nwk 

Nwk

bait

GST tag

nwk 
nwk 
Dap160 

Dap160

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nwk 
N-terminal region
sufficient binding region
aa 1-754

coordinates relative to nwk-PB

nwk 
SH3b domain residue W677
mutation disrupting interaction
aa 677

W677A, coordinates relative to nwk-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Rodal et al., 2008)
FBrf0205746-6.Y2H
Reference
Rodal et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nwk 

Nwk

bait

fused to GAL4 DNA-binding domain

nwk 
Dap160 

Dap160

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nwk 
SH3b domain residue W677
mutation disrupting interaction
aa 677

W677A, coordinates relative to nwk-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Rodal et al., 2008)

Mutation of SH3a domain (W531A) does not affect the interaction.

(Rodal et al., 2008)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Rodal et al., 2008)
FBrf0205746-7.PD.WB
Reference
Rodal et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dap160 

Dap160

bait

GST tag

Dap160 
nwk 

Nwk

prey

His tag

nwk 
nwk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dap160 
SH3 domains
sufficient binding region
aa 638-1097

coordinates relative to Dap160-PA

nwk 
N-terminal region
sufficient binding region
aa 1-754

coordinates relative to nwk-PB

nwk 
SH3b domain residue W677
mutation disrupting interaction
aa 677

W677A, coordinates relative to nwk-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Rodal et al., 2008)
FBrf0249586-1.PD.MW
Reference
Del Signore et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dap160 

Dap160

bait

GST tag

Dap160 
Dap160 
nwk 

Nwk

prey

[35]S label

nwk 
nwk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dap160 
SH3C domain
sufficient binding region
unspecified

coordinates relative to Dap160-PA

Dap160 
SH3D domain
sufficient binding region
unspecified

coordinates relative to Dap160-PA

nwk 
F-BAR domain
sufficient binding region
aa 1-428
nwk 
SH3b domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.

(Del Signore et al., 2021)

The nwk F-Bar interacts with Dap160 SH3C and SH3D domains. nwk SH3b domain only interacts with Dap160 SH3C domain.

(Del Signore et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)