FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Syt1, stnA
FlyBase ID
FBig0000108961
Interaction Network
Interactions Browser links
Syt1 network
stnA network
Reported Interactions
FBrf0218619-1.PD.WB
Reference
Soekmadji et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Syt1 

Syt1

bait

GST tag

Syt1 
Syt1 
Syt1 
stnA 

stnA

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Syt1 
C2B domain
sufficient binding region
aa 320-474

coordinates relative to Syt1-PA

Syt1 
C2B domain
mutation disrupting interaction
aa 320-383

coordinates relative to Syt1-PA

Syt1 
C2B domain
mutation increasing interaction
aa 454-474aa 436-474

coordinates relative to Syt1-PA

coordinates relative to Syt1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Soekmadji et al., 2012)

Binding enhanced by the addition of Ca2+. Syt1 mutation D414N,D416N abolishes enhanced binding with Ca2+.

(Soekmadji et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)