FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cam, Vha100-1
FlyBase ID
FBig0000109056
Interaction Network
Interactions Browser links
Cam network
Vha100-1 network
Reported Interactions
FBrf0203069-1.CH.MW
Reference
Zhang et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Vha100-1 

V100-N

neutral component
Vha100-1 

V100-N

neutral component
Vha100-1 
Vha100-1 
Vha100-1 

V100-N

neutral component
Vha100-1 
Vha100-1 
Cam 

CaM

neutral component
Cam 

CaM

neutral component
Cam 

CaM

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vha100-1 
N-terminal domain
sufficient binding region
aa 1-407
Vha100-1 
N-terminal domain
mutation disrupting interaction
aa 318aa 319

W318A

F319A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

(Zhang et al., 2008)
FBrf0203069-2.PD.MW
Reference
Zhang et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Vha100-1 

V100-N

bait

His tag

Vha100-1 
Vha100-1 
Cam 

CaM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vha100-1 
N-terminal domain
sufficient binding region
aa 1-407
Vha100-1 
N-terminal domain
mutation disrupting interaction
aa 318aa 319

W318A

F319A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Pull down assays to detect Vha100-1-Cam interaction included Ca2+.

(Zhang et al., 2008)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Zhang et al., 2008)
FBrf0203069-3.FS
Reference
Zhang et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Vha100-1 

V100-N

neutral component
Vha100-1 

V100-N

neutral component
Vha100-1 
Vha100-1 

V100-N

neutral component
Vha100-1 
Cam 

CaM

neutral component
Cam 

CaM

neutral component
Cam 

CaM

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vha100-1 
segment 2 domain
sufficient binding region
aa 314-338
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Kd = 21.5nM

(Zhang et al., 2008)

Interaction in vitro; peptide synthesized commercially;protein produced as recombinant fusion protein in bacterial system.

(Zhang et al., 2008)
FBrf0224656-4.BLI
Reference
Wang et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cam 

Cam

bait

His tag

Vha100-1 

Vha100-1

prey
Vha100-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vha100-1 
N-terminal domain
sufficient binding region
aa 1-141,164-407

coordinates relative to Vha100-1-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

(Wang et al., 2014)
FBrf0224656-7.PD.WB
Reference
Wang et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cam 

Cam

bait

GST tag

Vha100-1 

Vha100-1

prey
Vha100-1 
Vha100-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vha100-1 
N-terminal domain
sufficient binding region
aa 1-141,164-407

coordinates relative to Vha100-1-PC

Vha100-1 
Ca-CaM binding domain
mutation disrupting interaction
aa 318,319,328

coordinates relative to Vha100-1-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Wang et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)