FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000109191
Interaction Network
Interactions Browser links
Nrx-1 network
Ephrin network
Reported Interactions
FBrf0235199-1.Y2H
Reference
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Nrx-1

bait

fused to GAL4 DNA-binding domain

Ephrin

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
interaction domain
sufficient binding region
aa 1716-1837

coordinates relative to Nrx-1-PA

interaction domain
sufficient binding region
aa 605-652
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from unspecified cDNA expression library).

FBrf0235199-2.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Nrx-1

bait

Ephrin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was pupal retinae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0235199-3.PD.MW
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Nrx-1

bait

GST tag

Ephrin

prey

MBP label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
interaction domain
sufficient binding region
aa 1732-1813

coordinates relative to Nrx-1-PA

interaction domain
necessary binding region
aa 1760-1813

coordinates relative to Nrx-1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)