FB2020_06, released Dec 22, 2020

Physical Interaction report

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General Information
Interaction Type
physical association
Interacting Genes
mEFTu1, mt:tRNA:Lys-CTT
FlyBase ID
FBig0000109236
Interaction Network
Interactions Browser links
mEFTu1 network
mt:tRNA:Lys-CTT network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0234985-1.FP
Reference
Sato et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mEFTu1 

EF-Tu1

bait

His tag

mt:tRNA:Lys-CTT 

mt tRNA[Lys]

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

mEFTu1 binds cloverleaf tRNAs strongly (mt:tRNA:Lys-CTT, mt:tRNA:Cys-GCA) and D-armless tRNA comparatively weakly (mt:tRNA:Ser-GCT).

(Sato et al., 2017)

Interaction in vitro; bait produced as recombinant protein in bacterial system; prey produced by in vitro transcription.

(Sato et al., 2017)

Binding inferred by protection of tRNA from deacylation by protein.

(Sato et al., 2017)
External Crossreferences and Linkouts ( 0 )
References (1)