mEFTu1 binds cloverleaf tRNAs strongly (mt:tRNA:Lys-CTT, mt:tRNA:Cys-GCA) and D-armless tRNA comparatively weakly (mt:tRNA:Ser-GCT).
Interaction in vitro; bait produced as recombinant protein in bacterial system; prey produced by in vitro transcription.
Binding inferred by protection of tRNA from deacylation by protein.