FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000109242
Interaction Network
Interactions Browser links
Mcm3 network
Psf1 network
Reported Interactions
FBrf0214314-16.CS.EM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mcm3 

Mcm3

neutral component
Mcm3 

Mcm3

neutral component
Mcm3 

Mcm3

neutral component
Psf1 

Psf1

neutral component
Psf1 

Psf1

neutral component
Psf1 

Psf1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced in a baculovirus and insect cell system.

The CMG complex is captured as 55 pairwise direct or indirect interactions.

FBrf0214314-61.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mcm3 

Mcm3

bait

FLAG tag

Psf1 

Psf1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in a baculovirus and insect cell system; prey in a baculovirus and insect cell system.

FBrf0228822-7.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mcm3 

Mcm3

bait

FLAG tag

Psf1 

Psf1

prey

HA tag

Psf1 
Psf1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Psf1 
C-terminal domain
necessary binding region
aa 185-202
Psf1 
C-terminal domain
mutation disrupting interaction
aa 190,192,193,194

E190A,L192A,V193A,R194A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; recombinant proteins produced in baculovirus and insect cell system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)