FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000109278
Interaction Network
Interactions Browser links
osk network
cup network
Reported Interactions
FBrf0219299-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

osk

bait
cup 

cup

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0219299-3.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

osk

bait

fused to GAL4 DNA-binding domain

osk 
cup 

cup

prey

fused to GAL4 activation domain

cup 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
interaction domain
sufficient binding region
aa 122-605

coordinates relative to osk-PA, authors described region as aa 122-650, presumed to be a typo.

cup 
interaction domain
sufficient binding region
aa 29-962
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

FBrf0248759-3.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

osk

bait

FLAG tag

cup 

Cup

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)