FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
neur, E(spl)mα-BFM
FlyBase ID
FBig0000109432
Interaction Network
Interactions Browser links
neur network
E(spl)mα-BFM network
Reported Interactions
FBrf0190191-9.Y2H
Reference
Bardin and Schweisguth, 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
neur 

Neur

bait

fused to LexA DNA-binding domain

neur 
E(spl)mα-BFM 

mα

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
neur 
NHR domains
sufficient binding region
aa 93-532
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bardin and Schweisguth, 2006)

Two-hybrid system: yeast LexA-BD/B42-AD

(Bardin and Schweisguth, 2006)
FBrf0208547-1.PD.WB
Reference
Fontana and Posakony, 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
neur 

Neur

bait

GST tag

E(spl)mα-BFM 

E(spl)mα

prey

His tag

E(spl)mα-BFM 
E(spl)mα-BFM 
E(spl)mα-BFM 
E(spl)mα-BFM 
E(spl)mα-BFM 
E(spl)mα-BFM 
E(spl)mα-BFM 
E(spl)mα-BFM 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
E(spl)mα-BFM 
B domain
mutation decreasing interaction
aa 25-26,30,33

multiple alanine substitutions.

E(spl)mα-BFM 
B domain and N domain
mutation decreasing interaction
aa 25-26,30,33,68,71-74

multiple alanine substitutions.

E(spl)mα-BFM 
B domain and G2 domain
mutation decreasing interaction
aa 25-26,30,33,110,111,112

multiple alanine substitutions.

E(spl)mα-BFM 
Y domain
mutation disrupting interaction
aa 39,41-53

multiple glutamine substitutions.

E(spl)mα-BFM 
Z domain
mutation disrupting interaction
aa 54-55,57-67

multiple glutamine substitutions.

E(spl)mα-BFM 
N and N prime domains
mutation disrupting interaction
aa 51-54,56,68,71-74

multiple alanine substitutions.

E(spl)mα-BFM 
N motif
sufficient binding region
aa 63-80
E(spl)mα-BFM 
N motif
mutation disrupting interaction
aa 68,71

two alanine substitutions.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced produced as a recombinant fusion protein in bacterial system.

(Fontana and Posakony, 2009)

h does not bind to neu in his assay.

(Fontana and Posakony, 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)