FB2026_02 , released June 18, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
pros, Clbn
FlyBase ID
FBig0000109549
Interaction Network
Interactions Browser links
pros network
Clbn network
Reported Interactions
FBrf0191360-1.Y2H
Reference
Bi et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pros 

Prospero

bait

fused to GAL4 DNA-binding domain

pros 
Clbn 

Clbn

prey

fused to GAL4 activation domain

Clbn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pros 
homeodomain HD
sufficient binding region
Clbn 
200 amino acid carboxy terminal
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Bi et al., 2005)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bi et al., 2005)
FBrf0191360-2.Y2H
Reference
Bi et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pros 

Prospero

bait

fused to GAL4 DNA-binding domain

pros 
pros 
pros 
Clbn 

Clbn

prey

fused to GAL4 activation domain

Clbn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pros 
homeodomain HDA
sufficient binding region
Clbn 
200 amino acid carboxy terminal
sufficient binding region
pros 
HDA-F4
mutation decreasing interaction
pros 
HDA-LLL
mutation decreasing interaction
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bi et al., 2005)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bi et al., 2005)
FBrf0191360-3.PD.A
Reference
Bi et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pros 

Pros

bait

HA tag

pros 
Clbn 

Clbn

prey

MYC tag,[35]S label

Clbn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pros 
homeodomain HD
sufficient binding region
Clbn 
200 amino acid carboxy terminal
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Bi et al., 2005)
FBrf0191360-4.PD.A
Reference
Bi et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Clbn 

Clbn

bait

HA tag

Clbn 
pros 

Pros

prey

MYC tag,[35]S label

pros 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pros 
homeodomain HD
sufficient binding region
Clbn 
200 amino acid carboxy terminal
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Bi et al., 2005)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)