FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Ubx, 14-3-3ε
FlyBase ID
FBig0000109565
Interaction Network
Interactions Browser links
Ubx network
14-3-3ε network
Reported Interactions
FBrf0226424-6.Y2H
Reference
Hsiao et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait

fused to lexA DNA-binding domain

Ubx 
Ubx 
Ubx 
Ubx 
Ubx 
14-3-3ε 

14-3-3ε

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ubx 
interaction domain
sufficient binding region
aa 1-291

coordinates refer to Ubx-PA

Ubx 
interaction domain
necessary binding region
aa 1-215

coordinates refer to Ubx-PA

Ubx 
interaction domain
mutation decreasing interaction
aa 1-103aa 103-216aa 250-292

coordinates refer to Ubx-PA

coordinates refer to Ubx-PA

coordinates refer to Ubx-PA

Ubx 
interaction domain
mutation disrupting interaction
aa 1-103,250-292aa 1-103,292-389aa 103-216,250-292

coordinates refer to Ubx-PA

coordinates refer to Ubx-PA

coordinates refer to Ubx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Hsiao et al., 2014)

Transcriptionally inactive mutant A226P,Q233P of Ubx used.

(Hsiao et al., 2014)
FBrf0251367-1.coIP.WB
Reference
Wei et al., 2021
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
14-3-3ε 

14-3-3ε

bait
Ubx 

Ubx

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wei et al., 2021)
FBrf0251367-2.coIP.WB
Reference
Wei et al., 2021
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait
14-3-3ε 

14-3-3ε

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wei et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)