FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Shal, SIDL
FlyBase ID
FBig0000110070
Interaction Network
Interactions Browser links
Shal network
SIDL network
Reported Interactions
FBrf0211355-1.Y2H
Reference
Diao et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Shal 

Shal

bait

fused to GAL4 DNA-binding domain

Shal 
SIDL 

SIDL

prey

fused to GAL4 activation domain

SIDL 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Shal 
C-terminus
sufficient binding region
aa 411-571

coordinates relative to Shal-PB

SIDL 
C-terminus
sufficient binding region
aa 764-1145
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Diao et al., 2010)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Diao et al., 2010)
FBrf0211355-2.Y2H
Reference
Diao et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Shal 

Shal

bait

fused to GAL4 DNA-binding domain

Shal 
Shal 
Shal 
SIDL 

SIDL

prey

fused to GAL4 activation domain

SIDL 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Shal 
LL motif
sufficient binding region
aa 474-489

coordinates relative to Shal-PB

SIDL 
C-terminus
sufficient binding region
aa 764-1145
Shal 
LL motif
necessary binding region
aa 481-489

coordinates relative to Shal-PB

Shal 
LL motif
mutation decreasing interaction
aa 481,482

L481A,L482A mutation, coordinates relative to Shal-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Diao et al., 2010)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Diao et al., 2010)
FBrf0211355-3.PD.WB
Reference
Diao et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Shal 

Shal

bait

GST tag

Shal 
Shal 
SIDL 

SIDL

prey

MYC tag

SIDL 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
SIDL 
C-terminus
sufficient binding region
aa 760-1145
Shal 
LL motif
sufficient binding region
aa 411-490

coordinates relative to Shal-PB

Shal 
LL motif
necessary binding region
aa 481-489

coordinates relative to Shal-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Diao et al., 2010)
FBrf0211355-4.PD.WB
Reference
Diao et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
SIDL 

SIDL

bait

GST tag

SIDL 
Shal 

Shal

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
SIDL 
C-terminus
sufficient binding region
aa 760-1145
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryo membrane extract.

(Diao et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)