FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Unc-76, Syt1
FlyBase ID
FBig0000110393
Interaction Network
Interactions Browser links
Unc-76 network
Syt1 network
Reported Interactions
FBrf0206363-5.coIP.WB
Reference
Toda et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Unc-76 

UNC-76

bait

HA tag

Unc-76 
Unc-76 
Syt1 

Syt-1

prey

MYC tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Unc-76 
phosphorylation target
mutation increasing interaction
aa 143

S143D

Unc-76 
phosphorylation target
mutation disrupting interaction
aa 143

S143A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Toda et al., 2008)
FBrf0206363-6.FRET.FM
Reference
Toda et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Syt1 

Syt1

fluorescence donor

CFP tag

Syt1 

Syt1

fluorescence donor

CFP tag

Syt1 

Syt1

fluorescence donor

CFP tag

Unc-76 

Unc-76

fluorescence acceptor

YFP tag

Unc-76 

Unc-76

fluorescence acceptor

YFP tag

Unc-76 

Unc-76

fluorescence acceptor

YFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was COS7 cell line; fluorescence donor produced from tagged transfected construct; fluorescence acceptor produced from tagged transfected construct.

(Toda et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)