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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000112747
Interaction Network
Interactions Browser links
gcl network
tor network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0236130-5.AP.MS
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gcl 

GCL

bait

FLAG tag

gcl 
tor 

Torso

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gcl 
C-terminal domain
necessary binding region
aa 253-569

coordinates relative to gcl-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0236130-6.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gcl 

GCL

bait

FLAG tag

tor 

Torso

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

Interaction increased by coexpression of a dominant negative form of Cul3.

FBrf0236130-7.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gcl 

GCL

bait

FLAG tag

gcl 
gcl 
gcl 
gcl 
tor 

Torso

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gcl 
BTB domain residue 100
mutation increasing interaction
aa 100

E100K, coordinates relative to gcl-PA

gcl 
GCL domain
necessary binding region
aa 363-402

coordinates relative to gcl-PA

gcl 
GCL domain residue 377
mutation disrupting interaction
aa 377

R377A, coordinates relative to gcl-PA

gcl 
MYR domain residue 2
mutation disrupting interaction
aa 2

G2A, coordinates relative to gcl-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0236130-8.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gcl 

GCL

bait

FLAG tag

gcl 
gcl 
gcl 
gcl 
gcl 
gcl 
gcl 
gcl 
tor 

Torso

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gcl 
GCL domain residue 377
mutation disrupting interaction
aa 377

R377A, coordinates relative to gcl-PA

gcl 
GCL domain residue 380
mutation disrupting interaction
aa 380

R380A, coordinates relative to gcl-PA

gcl 
GCL domain residue 382
mutation disrupting interaction
aa 382

L382A, coordinates relative to gcl-PA

gcl 
GCL domain residue 391
mutation disrupting interaction
aa 391

R391A, coordinates relative to gcl-PA

gcl 
GCL domain residue 394
mutation disrupting interaction
aa 394

G394A, coordinates relative to gcl-PA

gcl 
GCL domain residue 395
mutation disrupting interaction
aa 395

F395A, coordinates relative to gcl-PA

gcl 
GCL domain residue 400
mutation disrupting interaction
aa 400

D400A, coordinates relative to gcl-PA

gcl 
GCL domain residue 401
mutation decreasing interaction
aa 401

L401A, coordinates relative to gcl-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced by in vitro translation; prey produced by in vitro translation.

FBrf0236130-9.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tor 

Torso

bait

HA tag

tor 
tor 
tor 
tor 
tor 
tor 
gcl 

GCL

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tor 
degron domain
necessary binding region
aa 646-655

coordinates relative to tor-PA

tor 
degron domain residue 649
mutation disrupting interaction
aa 649

I649A, coordinates relative to tor-PA

tor 
degron domain residue 652
mutation disrupting interaction
aa 652

I652A, coordinates relative to tor-PA

tor 
degron domain residue 651
mutation decreasing interaction
aa 651

N651A, coordinates relative to tor-PA

tor 
degron domain residue 653
mutation decreasing interaction
aa 653

G653A, coordinates relative to tor-PA

tor 
degron domain
mutation disrupting interaction
aa 649-653

IENIG to AAAAA, coordinates relative to tor-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)