FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Fmr1, BicD
FlyBase ID
FBig0000113422
Interaction Network
Interactions Browser links
Fmr1 network
BicD network
Reported Interactions
FBrf0211628-1.PD.MS
Reference
Bianco et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BicD 

BicD

bait

GST tag

BicD 
BicD 
Fmr1 

FMRP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
BicD 
C-terminal domain
sufficient binding region
aa 538-782

coordinates relative to BicD-PA

BicD 
C-terminal domain residue 730
mutation disrupting interaction
aa 730

K730M, coordinates relative to BicD-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Bianco et al., 2010)
FBrf0211628-2.PD.WB
Reference
Bianco et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BicD 

BicD

bait

GST tag

BicD 
BicD 
Fmr1 

FMRP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
BicD 
C-terminal domain
sufficient binding region
aa 538-782

coordinates relative to BicD-PA

BicD 
C-terminal domain residue 730
mutation disrupting interaction
aa 730

K730M, coordinates relative to BicD-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Bianco et al., 2010)
FBrf0211628-3.coIP.WB
Reference
Bianco et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BicD 

BicD

bait
Fmr1 

FMRP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Bianco et al., 2010)
FBrf0211628-8.Y2H
Reference
Bianco et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

FMRP

bait

fused to GAL4 DNA-binding domain

Fmr1 
BicD 

BicD

prey

fused to GAL4 activation domain

BicD 
BicD 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Fmr1 
interaction domain
sufficient binding region
aa 220-681

coordinates relative to Fmr1-PC

BicD 
C-terminal domain
sufficient binding region
aa 538-782

coordinates relative to BicD-PA

BicD 
C-terminal domain residue 730
mutation disrupting interaction
aa 730

K730M, coordinates relative to BicD-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bianco et al., 2010)

Two-hybrid system: yeast GAL4-BD/GAL4-AD, The Fmr1-BicD interaction was not detected by two-hybrid using full length Fmr1, but could be detected using the aa 220-681 fragment of Fmr1.

(Bianco et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)