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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000113560
Interaction Network
Interactions Browser links
p53 network
Daxx network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0202871-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
p53 

Dmp53

bait

fused to LexA DNA-binding domain

p53 
p53 
Daxx 

DLP

prey

fused to B42 activation domain

Daxx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Daxx 
C terminal domain
sufficient binding region
aa 1524-1659
p53 
C terminal domain
sufficient binding region
aa 277-385

coordinates relative to p53-PA

p53 
C terminal domain
necessary binding region
aa 295-385
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast LexA-BD/B42-AD

FBrf0202871-2.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
p53 

p53

bait

GST tag

p53 
Daxx 

DLP

prey

[3]H-leucine label

Daxx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Daxx 
C terminal domain
sufficient binding region
aa 1524-1659
p53 
C terminal domain
sufficient binding region
aa 277-385

coordinates relative to p53-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0210534-40.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
p53 

Dmp53

bait

MBP tag

Daxx 

CG9537

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and Sf9 cell system; prey produced and labeled by in vitro translation.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)