Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.
The mass spectrum of mira shows monomeric and dimeric forms.
Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.
Wild-type mira runs as a dimer on non-reducing SDS-PAGE. The C476S mutant form runs as a monomer suggesting disulfide bridge formation.
Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.
A band consistent with a dimer emerges on SDS-PAGE with increasing concentrations of cross-linking reagent.
Source was larval brains of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.
MS2 RNA tag was detected by coexpression of MCP-GFP (MCP binds to MS2 RNA) and anti-GFP western blot.
