FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Mad, shn
FlyBase ID
FBig0000114212
Interaction Network
Interactions Browser links
Mad network
shn network
Reported Interactions
FBrf0131280-1.Y2H
Reference
Dai et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
shn 

Shn

bait

fused to LexA DNA-binding domain

Mad 

Mad

prey

fused to B42 activation domain

Mad 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mad 
Mad homology region 2 with the linker region
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Dai et al., 2000)

Two-hybrid system: yeast LexA-BD/B42-AD

(Dai et al., 2000)
FBrf0131280-2.coIP.WB
Reference
Dai et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad 

Mad

bait

FLAG tag

shn 

Shn

prey

MYC tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS cell line; bait produced from transfected construct; prey produced from transfected construct.

(Dai et al., 2000)
FBrf0131280-3.PD.A
Reference
Dai et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad 

Mad

bait

GST tag

shn 

Shn

prey

[35]S label

shn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
shn 
Mad interaction domain
sufficient binding region
aa 341-1069aa 1441-1635aa 1776-2529

coordinates relative to a 2529 isoform reported in FBrf0082051.

coordinates relative to a 2529 isoform reported in FBrf0082051.

coordinates relative to a 2529 isoform reported in FBrf0082051.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Dai et al., 2000)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)