FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
msl-3, His4
FlyBase ID
FBig0000115893
Interaction Network
Interactions Browser links
msl-3 network
His4 network
Reported Interactions
FBrf0212637-1.SPR
Reference
Moore et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msl-3 

dMSL3

bait

GST tag

msl-3 
His4 

H4

prey
His4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
msl-3 
chromo-barrel domain
sufficient binding region
aa 2-91
His4 
interaction domain
sufficient binding region
GKGGAKRHRKVLRDY

mono-, di-, or trimethylated at K20

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide chemically synthesized in vitro.

(Moore et al., 2010)

Binding preference H4K20me2 > H4K20me1 > H4K20me3.

(Moore et al., 2010)
FBrf0214093-1.FPS
Reference
Kim et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msl-3 

MSL3

bait

His tag

msl-3 
His4 

H4

prey

fluorescein label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
msl-3 
Chromo domain
sufficient binding region
aa 1-90
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Kd=10uM

(Kim et al., 2010)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide chemically synthesized and labeled in vitro.

(Kim et al., 2010)
External Crossreferences and Linkouts ( 0 )
References (2)