FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nlg4, tkv
FlyBase ID
FBig0000115996
Interaction Network
Interactions Browser links
Nlg4 network
tkv network
Reported Interactions
FBrf0237099-1.coIP.WB
Reference
Zhang et al., 2017
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tkv 

Tkv

bait

GFP tag

Nlg4 

DNlg4

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Zhang et al., 2017)
FBrf0237099-2.coIP.WB
Reference
Zhang et al., 2017
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nlg4 

DNlg4

bait
tkv 

Tkv

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Zhang et al., 2017)
FBrf0237099-3.PD.WB
Reference
Zhang et al., 2017
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nlg4 

DNlg4

bait

GST or His tag

Nlg4 
Nlg4 
tkv 

Tkv

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nlg4 
N-terminal domain
sufficient binding region
aa 1-675

coordinates relative to Nlg4-PD

Nlg4 
N-terminal domain
necessary binding region
aa 1-847

coordinates relative to Nlg4-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Zhang et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)