FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
LKRSDH, EcR
FlyBase ID
FBig0000116014
Interaction Network
Interactions Browser links
LKRSDH network
EcR network
Reported Interactions
FBrf0205793-1.PD.MS
Reference
Cakouros et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
mbn2
Participants
Corresponds to
Reported as
Role
Note
EcR 

EcR

bait

bound to biotin-tagged oligonucleotide with EcR/usp binding site

LKRSDH 

dLKR/SDH

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait endogenous to nuclear extract.

(Cakouros et al., 2008)

Prey endogenous to nuclear extract.

(Cakouros et al., 2008)

Nuclear extracts from ecdysone-treated cells were incubated with a biotin-tagged oligonucleotide corresponding to the EcR/usp DNA-binding site of the Nc promoter.

(Cakouros et al., 2008)
FBrf0205793-2.PD.A
Reference
Cakouros et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
LKRSDH 

SDH

bait

GST tag

LKRSDH 
LKRSDH 
EcR 

EcR-B1

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
LKRSDH 
SDH domain
sufficient binding region
aa 618–928
LKRSDH 
SDH domain
mutation decreasing interaction
aa 618–802

deletion weakens binding

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

(Cakouros et al., 2008)

Prey derived from in vitro translation.

(Cakouros et al., 2008)
FBrf0205793-3.coIP.WB
Reference
Cakouros et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
mbn2
Participants
Corresponds to
Reported as
Role
Note
EcR 

EcR-B1

bait
LKRSDH 

dLKR/SDH

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait endogenous to cells.

(Cakouros et al., 2008)

Prey endogenous to cells.

(Cakouros et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)