FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000116041
Interaction Network
Interactions Browser links
Shal network
SKIP network
Reported Interactions
FBrf0208394-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Shal 

Shal1

bait

fused to GAL4 DNA-binding domain

Shal 
SKIP 

SKIP3

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Shal 
C-terminal domain
sufficient binding region
aa 411-571

coordinates relative to Shal-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0208394-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Shal 

Shal1

bait

fused to GAL4 DNA-binding domain

Shal 
SKIP 

SKIP3

prey

fused to GAL4 activation domain

SKIP 
SKIP 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Shal 
C-terminal domain
sufficient binding region
aa 411-571aa 451-490

coordinates relative to Shal-PB

coordinates relative to Shal-PA, reported as Shal2

SKIP 
C-terminal domain
sufficient binding region
aa 67-128

coordinates relative to SKIP-PH, reported as SKIP3

SKIP 
C-terminal domain
necessary binding region
aa 121-128

coordinates relative to SKIP-PH, reported as SKIP3

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0208394-3.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Shal 

Shal

bait

GST tag

Shal 
SKIP 

SKIP3

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Shal 
C-terminal domain
sufficient binding region
aa 411-571aa 411-490

coordinates relative to Shal-PB, reported as Shal1

coordinates relative to Shal-PA, reported as Shal2

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)