Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from universal cDNA expression library- Clontech, 630485).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type or mutant adult head extract.
