FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pum, GluRIIA
FlyBase ID
FBig0000116358
Interaction Network
Interactions Browser links
pum network
GluRIIA network
Reported Interactions
FBrf0215373-1.EMSA.A
Reference
Menon et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pum 

Pum

bait

His tag

pum 
GluRIIA 

GluRIIA

prey

[32]P label

GluRIIA 
GluRIIA 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
GluRIIA 
3-prime UTR
sufficient binding region
GAUCGGUGUCCACAUUGUAUAUUGUGAGCUAUAUUUGUAGAAUUAUUUU
GluRIIA 
3-prime UTR
mutation decreasing interaction
GAUCGGUGUCCACAUUGUAUAUUGUGAGCUAUAUUUGUAGAAUUAUUUUGAUCGGUGUCCACAUUGUAUAUUGUGAGCUAUAUUUGUAGAAUUAUUUU

two UGUA motifs mutated to GUUA

AUUGUGA to CUGAGAC

pum 
RNA-binding domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

(Menon et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)