Source was yeast cell line; bait produced from DNA containing 3\'UTR of bcd; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Three-hybrid system: yeast RevM10-GAL4-BD/GAL4-AD
A419G, A427C, C428A, numbering starts with the first nucleotide after the Stop codon
Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.
