FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Pits, ttk
FlyBase ID
FBig0000116624
Interaction Network
Interactions Browser links
Pits network
ttk network
Reported Interactions
FBrf0238907-1.Y2H
Reference
Liaw, 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ttk 

Ttk69

bait

fused to GAL4 DNA-binding domain

Pits 

CG11138

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Liaw, 2016)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Liaw, 2016)
FBrf0238907-2.PD.WB
Reference
Liaw, 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pits 

CG11138

bait

GST tag

Pits 
Pits 
ttk 

Ttk69

prey

S or His tag

ttk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pits 
C-terminal domain
sufficient binding region
aa 434-630

coordinates relative to Pits-PD, binds to full length ttk.

Pits 
middle domain
sufficient binding region
aa 160-406

coordinates relative to Pits-PD, binds to N-terminal fragment of ttk, aa 5-218.

ttk 
N-terminal domain
sufficient binding region
aa 5-218

coordinates relative to ttk-PA, binds to full length Pits and the middle fragment of Pits, aa 160-406.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Liaw, 2016)
FBrf0238907-4.coIP.WB
Reference
Liaw, 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pits 

CG11138

bait
ttk 

Ttk69

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Liaw, 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)