FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000116650
Interaction Network
Interactions Browser links
Cdc6 network
Orc2 network
Reported Interactions
FBrf0239330-28.PD.MW
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdc6 

Cdc6

bait

MBP tag

Orc2 

Orc2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Deletion of the Orc1 N-terminus decreases the ability of Cdc6 to pull down the Orc complex.

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and High5 cell system.

FBrf0246581-2.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdc6 

Cdc6

bait

MBP tag

Cdc6 
Orc2 

Orc2

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cdc6 
interaction domain
sufficient binding region
aa 242-662

coordinates relative to Cdc6-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cdc6-ORC interactions were detected in the context of the ORC complex bound to DNA.

Interaction in vitro; bait produced as a recombinant fusion protein in a baculovirus and insect cell system; prey produced as a recombinant fusion protein in a baculovirus and insect cell system.

FBrf0246581-8.EM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdc6 

Cdc6

neutral component
Cdc6 
Cdc6 

Cdc6

neutral component
Cdc6 
Cdc6 

Cdc6

neutral component
Orc2 

Orc2

neutral component
Orc2 

Orc2

neutral component
Orc2 

Orc2

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cdc6 
interaction domain
sufficient binding region
aa 242-662

coordinates relative to Cdc6-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in a baculovirus and insect cell system.

Comparison of EM structures of ORC complex, ORC complex bound to DNA and a ternary complex consisting of ORC, DNA and Cdc6 curated as a series on binary protein-protein interactions.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)