FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
para, pum
FlyBase ID
FBig0000116701
Interaction Network
Interactions Browser links
para network
pum network
Reported Interactions
FBrf0203129-1.coIP.PCR
Reference
Muraro et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pum 

Pum

bait

TAP tag

pum 
para 

para

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pum 
N-terminal domain
sufficient binding region
aa 1-524

coordinates relative to pum-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult flies; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Muraro et al., 2008)
FBrf0203129-2.PD.WB
Reference
Muraro et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
para 

para

bait

biotin label

pum 

Pum

prey

TAP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The para-pum interaction can be competed with RNA containing the pum binding motif - AUUGUAAAUA but not with a mutant RNA - AUACAAAAUA

(Muraro et al., 2008)

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from adult fly extract.

(Muraro et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)