FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
CSN3, Mlf
FlyBase ID
FBig0000116738
Interaction Network
Interactions Browser links
CSN3 network
Mlf network
Reported Interactions
FBrf0202909-1.coIP.WB
Reference
Sugano et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc-unspecified
Participants
Corresponds to
Reported as
Role
Note
CSN3 

dCSN3

bait

HA tag

Mlf 

dMLF

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sugano et al., 2008)
FBrf0202909-2.PD.A
Reference
Sugano et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CSN3 

dCSN3

bait

GST tag

CSN3 
CSN3 
Mlf 

dMLF

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CSN3 
PCI domain
sufficient binding region
aa 211-378
CSN3 
N-terminal domain
sufficient binding region
aa 1-81

binds more weakly than full length

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Sugano et al., 2008)
FBrf0202909-3.PD.A
Reference
Sugano et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mlf 

dMLF

bait

GST tag

Mlf 
Mlf 
CSN3 

dCSN3

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mlf 
N-terminal domain
sufficient binding region
aa 1-202
Mlf 
N-terminal domain
necessary binding region
aa 1-202
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Sugano et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)