Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from larval cDNA expression library).
Two-hybrid system: yeast LexA-BD/GAL4-AD
YPWM to AAAA, coordinates relative to Antp-PJ
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast LexA-BD/GAL4-AD
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
YPWM to AAAA, coordinates relative to Antp-PJ
Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.
