Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA library)
Interaction between Acn and Aac11 was further studied and supported using the human proteins in mammalian cells by coimmunoprecipitation.
Several Acn fragments were identified two times in the screen.
Two-hybrid system; yeast GAL4-BD/GAL4-AD