FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
TfIIEβ, Antp
FlyBase ID
FBig0000116850
Interaction Network
Interactions Browser links
TfIIEβ network
Antp network
Reported Interactions
FBrf0240335-1.BiFC.FM
Reference
Altamirano-Torres et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
TfIIEβ 

TFIIEβ

bait

Venus tag, N-terminal fragment

Antp 

Antp

prey

Venus tag, C-terminal fragment

Antp 
Antp 
Antp 
Antp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Antp 
H2 domain
necessary binding region
unspecified
Antp 
H2 domain residue 32
mutation decreasing interaction
aa 32

I32A or I32L

Antp 
H2 domain residue 36
mutation decreasing interaction
aa 36

H36A or H36R

Antp 
H2 domain residue 32,36
mutation disrupting interaction
aa 32,36

I32A,H36A or I32L,H36R

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live HEK293 cells; proteins produced from transfected constructs.

(Altamirano-Torres et al., 2018)
FBrf0240335-2.BiFC.FM
Reference
Altamirano-Torres et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
TfIIEβ 

TFIIEβ

bait

Venus tag, N-terminal fragment

Antp 

Antp

prey

Venus tag, C-terminal fragment

Antp 
Antp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Antp 
C-terminal domain
sufficient binding region
unspecified
Antp 
H2 domain residue 32,36
mutation disrupting interaction
aa 32,36

I32A,H36A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Altamirano-Torres et al., 2018)
FBrf0240335-3.BiFC.FM
Reference
Altamirano-Torres et al., 2018
Description
physical association
Assay
Collection
Source/Stage
imaginal disc / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
TfIIEβ 

TFIIEβ

bait

Venus tag, N-terminal fragment

Antp 

Antp

prey

Venus tag, C-terminal fragment

Antp 
Antp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Antp 
C-terminal domain
sufficient binding region
unspecified
Antp 
H2 domain residue 32,36
mutation disrupting interaction
aa 32,36

I32A,H36A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was imaginal discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Altamirano-Torres et al., 2018)
FBrf0253692-2.FRET.FM
Reference
Jiménez-Mejía et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
TfIIEβ 

TfIIEβ

fluorescence donor

ECFP tag

TfIIEβ 

TfIIEβ

fluorescence donor

ECFP tag

TfIIEβ 

TfIIEβ

fluorescence donor

ECFP tag

Antp 
Antp 
Antp 

Antp

fluorescence acceptor

YFP tag

Antp 

Antp

fluorescence acceptor

YFP tag

Antp 
Antp 
Antp 

Antp

fluorescence acceptor

YFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Antp 
N terminal domain residues 1-269
mutation disrupting interaction
aa 1-269

deletion mutant

Antp 
HD helix domain residues 328 and 332
mutation disrupting interaction
aa 328 and 332

I328A, H332A.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was HEK293 cell line; fluorescence donor produced from tagged transgenic construct; fluorescence acceptor produced from tagged transgenic construct.

(Jiménez-Mejía et al., 2022)

Published coordinates are relative to the HD domain.

(Jiménez-Mejía et al., 2022)

Antp was fused to Hsap\TBP and formed a trimer with TfIIEβ.

(Jiménez-Mejía et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (2)