FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
nod, Eb1
FlyBase ID
FBig0000116945
Interaction Network
Interactions Browser links
nod network
Eb1 network
Reported Interactions
FBrf0239997-1.PD.A
Reference
Ye et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nod 

NOD

bait

MBP, GFP tag and dimerization domain of human kinesin-1

nod 
Eb1 

EB1

prey

GST, TagRFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nod 
interaction domain
sufficient binding region
aa 1-485
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Ye et al., 2018)
FBrf0239997-2.PA.WB
Reference
Ye et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nod 

NOD

bait
nod 
nod 
nod 
nod 
Eb1 

EB1

prey

GST, TagRFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nod 
PT1 domain
sufficient binding region
aa 434-448
nod 
PT1 domain residue 434
mutation disrupting interaction
aa 434

R434A

nod 
PT2 domain
sufficient binding region
aa 472-484
nod 
PT2 domain residue 482
mutation disrupting interaction
aa 482

R482A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait synthesized in vitro; prey produced as a recombinant fusion protein in bacterial system.

(Ye et al., 2018)
FBrf0239997-3.MST
Reference
Ye et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nod 

NOD

bait

FITC label

nod 
Eb1 

EB1

prey

GST, TagRFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nod 
PT1 domain
sufficient binding region
aa 434-448
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Kd = 725nM

(Ye et al., 2018)

Interaction in vitro; bait synthesized in vitro; prey produced as a recombinant fusion protein in bacterial system.

(Ye et al., 2018)
External Crossreferences and Linkouts ( 0 )
References (1)