FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Ubx, Antp
FlyBase ID
FBig0000116995
Interaction Network
Interactions Browser links
Ubx network
Antp network
Reported Interactions
FBrf0240351-255.BiFC.FM
Reference
Bischof et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait

YFP tag, N-terminal fragment

Antp 

Antp

prey

CFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; proteins produced from tagged transgenic constructs.

(Bischof et al., 2018)
FBrf0260147-1.BiFC.FM
Reference
Villarreal-Puente et al., 2024
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stageeye disc / third instar larval stagewing disc / third instar larval stageleg disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait

Venus tag, N-terminal fragment

Ubx 
Antp 

Antp

prey

Venus tag, C-terminal fragment

Antp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ubx 
Homeodomain
sufficient binding region
aa 295-355
Antp 
Homeodomain
sufficient binding region
aa 245-363
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos and eye, wing and leg imaginal discs of transgenic fly line; proteins produced from tagged transgenic constructs.

(Villarreal-Puente et al., 2024)
FBrf0260147-2.BiFC.FM
Reference
Villarreal-Puente et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait

Venus tag, N-terminal fragment

Ubx 
Antp 

Antp

prey

Venus tag, C-terminal fragment

Antp 
Antp 
Antp 
Antp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ubx 
Homeodomain
sufficient binding region
aa 245-363
Antp 
Homeodomain
sufficient binding region
aa 245-363
Antp 
Homeodomain helix 1
mutation decreasing interaction
aa 308-322
Antp 
Homeodomain helix 1 and 2
mutation decreasing interaction
aa 308-334
Antp 
Homeodomain motif residue 315
mutation decreasing interaction
aa 315

E315G

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Deletion of Antp homeodomain helix 1 or both helix 1 and 2 decreases interaction with Ubx to 24 percent and 12 percent respectively. Antp E315G decreases interaction with Ubx to 60 percent.

(Villarreal-Puente et al., 2024)

Source was live HEK293 cells; proteins produced from tagged transgenic constructs.

(Villarreal-Puente et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (2)