FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
aop, Fbl6
FlyBase ID
FBig0000117014
Interaction Network
Interactions Browser links
aop network
Fbl6 network
Reported Interactions
FBrf0205459-1.Y2H
Reference
Roukens et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aop 

Yan

bait

fused to GAL4 DNA-binding domain

Fbl6 

Fbl6

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Roukens et al., 2008)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Roukens et al., 2008)
FBrf0205459-2.coIP.WB
Reference
Roukens et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fbl6 

Fbl6

bait

V5 tag

Fbl6 
Fbl6 
aop 

Yan

prey

HA tag

aop 
aop 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aop 
SAM domain
necessary binding region
unspecified
aop 
SAM domain residue 86
mutation disrupting interaction
aa 86

R86A

Fbl6 
F-box domain
mutation decreasing interaction
unspecified

deletion of F-box domain

Fbl6 
LRD2 domain
mutation decreasing interaction
unspecified

deletion of LRD2 domain

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Roukens et al., 2008)
External Crossreferences and Linkouts ( 0 )
References (1)