FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
aly, tomb
FlyBase ID
FBig0000117039
Interaction Network
Interactions Browser links
aly network
tomb network
Reported Interactions
FBrf0194011-1.Y2H
Reference
Jiang et al., 2007
Description
physical association
Assay
Collection
Source/Stage
testis / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aly 

Aly

bait

fused to Gal4 DNA-binding domain

aly 
tomb 

tomb

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aly 
interaction domain
sufficient binding region
aa 275-534
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from adult testis cDNA expression library).

(Jiang et al., 2007)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Jiang et al., 2007)
FBrf0194011-2.coIP.WB
Reference
Jiang et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aly 

Aly

bait

FLAG tag

tomb 

Tomb

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jiang et al., 2007)
FBrf0194011-3.coIP.WB
Reference
Jiang et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tomb 

Tomb

bait

HA tag

aly 

Aly

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jiang et al., 2007)
FBrf0194011-4.coIP.WB
Reference
Jiang et al., 2007
Description
physical association
Assay
Collection
Source/Stage
testis / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tomb 

Tomb

bait

EGFP tag

aly 

Aly

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult males of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Jiang et al., 2007)
FBrf0194011-5.Y2H
Reference
Jiang et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aly 

Aly

bait

fused to GAL4 DNA-binding domain

tomb 

Tomb

prey

fused to GAL4 activation domain

tomb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tomb 
Aly interacting domain
sufficient binding region
aa 136-243
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Jiang et al., 2007)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Jiang et al., 2007)
External Crossreferences and Linkouts ( 0 )
References (1)