FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nrx-IV, vari
FlyBase ID
FBig0000117395
Interaction Network
Interactions Browser links
Nrx-IV network
vari network
Reported Interactions
FBrf0215898-1.PD.WB
Reference
Bachmann et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nrx-IV 

NrxIV

bait

GST tag

Nrx-IV 
Nrx-IV 
vari 

Vari

prey

His tag

vari 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nrx-IV 
intracellular domain
sufficient binding region
aa 1235-1284

coordinates relative to Nrx-IV-PA

Nrx-IV 
PDZ-binding domain
necessary binding region
aa 1283-1284

coordinates relative to Nrx-IV-PA

vari 
PDZ domain
sufficient binding region
aa 169-270

coordinates relative to vari-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Bachmann et al., 2008)
FBrf0215898-2.coIP.WB
Reference
Bachmann et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nrx-IV 

NrxIV

bait

GFP tag

vari 

Vari

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Bachmann et al., 2008)

Both long and short isoforms of vari coprecipitate with Nrx-IV.

(Bachmann et al., 2008)
External Crossreferences and Linkouts ( 0 )
References (1)