Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

insv protein forms dimers, tetramers and possibly hexamers in solution.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

Crosslinking insv protein yields bands consistent with dimers and tetramers on SDS-PAGE.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD