FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000118615
Interaction Network
Interactions Browser links
M1BP network
Reported Interactions
FBrf0241977-328.Y2H
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
M1BP 

M1BP

bait

fused to GAL4 DNA-binding domain

M1BP 

M1BP

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0248212-35.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
M1BP 

CG9797

bait

fused to GAL4 DNA-binding domain

M1BP 

CG9797

prey

fused to GAL4 activation domain

M1BP 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
M1BP 
Zn finger domain
sufficient binding region
aa 9-114
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

FBrf0253937-10.XC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
M1BP 

M1BP

self
M1BP 

M1BP

self
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Crystal structure of M1BP as a homodimer.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

FBrf0253937-14.CL.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
M1BP 

M1BP synonym

self

TRX-His tag

M1BP 
M1BP 

M1BP synonym

self

TRX-His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
M1BP 
Zinc finger associated domain residues 2-98
sufficient binding region
aa 2-98
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; Protein produced as a recombinant fusion protein in bacterial system

FBrf0253937-5.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
M1BP 

M1BP

self

MBP tag

M1BP 
M1BP 

M1BP

self

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
M1BP 
Zinc finger associated domain residues 2-98
sufficient binding region
aa 2-98
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; Protein produced as a recombinant fusion protein in bacterial system.

FBrf0253937-7.CH
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
M1BP 

M1BP

self
M1BP 

M1BP

self
M1BP 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
M1BP 
Zinc finger associated domain residues 40 and 66
sufficient binding region
aa 40 and 66

I40A, L66S

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

FBrf0259088-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
M1BP 

M1BP

bait

fused to GAL4 DNA-binding domain

M1BP 

M1BP

prey

fused to GAL4 activation domain

M1BP 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
M1BP 
ZAD domain
sufficient binding region
aa 12-83

coordinates relative to M1BP-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

External Crossreferences and Linkouts ( 0 )
References (4)