Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-Sf9 cell system; prey derived from in vitro transcription of random primed genomic DNA PCR products.

B52-bound RNA was isolated by six rounds of SELEX and cDNAs were cloned. SELEX clones that mapped to the intron-containing genes were reported and further characterized.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-Sf9 cell system; prey produced and labeled by in vitro transcription.

B52-bound RNA was isolated by six rounds of SELEX and cDNAs were cloned.