Source was adult female ovaries; bait produced and labeled by in vitro transcription; prey produced as transgenic fusion protein in bacteriophage (prey from adult ovary cDNA expression library).
Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.