FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
comr, topi
FlyBase ID
FBig0000119838
Interaction Network
Interactions Browser links
comr network
topi network
Reported Interactions
FBrf0174565-1.Y2H
Reference
Perezgasga et al., 2004
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
comr 

Comr

bait

fused to GAL4 DNA-binding domain

topi 

Topi

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Perezgasga et al., 2004)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from testis cDNA expression library).

(Perezgasga et al., 2004)
FBrf0174565-2.Y2H
Reference
Perezgasga et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
comr 

Comr

bait

fused to GAL4 DNA-binding domain

comr 
comr 
topi 

Topi

prey

fused to GAL4 activation domain

topi 
topi 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
comr 
interaction domain
sufficient binding region
aa 200-600aa 1-300

coordinates are approximate

coordinates are approximate

comr 
interaction domain
necessary binding region
aa 200-300

coordinates are approximate

topi 
Zn-finger domains
sufficient binding region
aa 200-500aa 500-814

coordinates are approximate

coordinates are approximate

topi 
Zn-finger domains
necessary binding region
aa 200-350aa 500-650

coordinates are approximate

coordinates are approximate

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Perezgasga et al., 2004)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.

(Perezgasga et al., 2004)
FBrf0174565-3.coIP.WB
Reference
Perezgasga et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
comr 

Comr

bait

FLAG tag

topi 

Topi

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Perezgasga et al., 2004)
FBrf0174565-4.coIP.WB
Reference
Perezgasga et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
topi 

Topi

bait

HA tag

comr 

Comr

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Perezgasga et al., 2004)
External Crossreferences and Linkouts ( 0 )
References (1)