Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Source was cell extract of HeLa cell line; bait produced from transfected construct; prey produced from transfected construct.
