The 14-3-3 antibody used was raised to the N-terminal 20 aa of human 14-3-3. This antibody may recognize both 14-3-3ε and 14-3-3ζ.

Source was wing discs of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Source was larvae of wild-type fly line; proteins produced from endogenous genes.

The 14-3-3 antibody used was raised to the N-terminal 20 aa of human 14-3-3. This antibody may recognize both 14-3-3ε and 14-3-3ζ.