Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD. A mutant version of Gem3 with its N-terminal helicase domain deleted dimerizes very weakly but binds to full-length Gem3 more strongly than full-length Gem3 binds to itself.