FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Polr2A, Pcif1
FlyBase ID
FBig0000120572
Interaction Network
Interactions Browser links
Polr2A network
Pcif1 network
Reported Interactions
FBrf0246474-1.PD.MW
Reference
Pandey et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Polr2A 

RNA PolII CTD

bait

biotin tag

Polr2A 
Pcif1 

dPcif1

prey

GST tag

Pcif1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pcif1 
WW domain
sufficient binding region
aa 138-172
Polr2A 
CTD domain
sufficient binding region

YSPTSPS repeat peptide

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

A Pcif1-RpII215 interaction was only detected using the WW domain and not with full length Pcif1.

(Pandey et al., 2020)

Interaction in vitro; bait was commercial synthetic peptide; prey produced as a recombinant fusion protein in a baculovirus and insect cell system.

(Pandey et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)